Análises Laboratoriais

Higiene e Segurança nos Laboratórios

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Publication in the Diário da República: Despacho nº 7835/2019 de 05/09/2019

3 ECTS; 1º Ano, 2º Semestre, 30,0 TP , Cód. 608010.

Lecturer
- Paula Alexandra Geraldes Portugal (1)(2)

(1) Docente Responsável
(2) Docente que lecciona

Prerequisites
Not applicable

Objectives
Students should be able to analyze and assess occupational health and safety risks in a laboratory environment, particularly chemical risks, as well as facility safety risks, and also be able to adopt the legally required or recommended measures for their control.

Program
1. Introduction
1.1 - Hazard and Risk
1.2 - Occupational Safety and Health
1.3 - Risk Categories - Physical, Chemical, Biological, and Ergonomic
1.4 - Effects of Exposure to Risks

2. Risk Management
2.1 - Domino Theory (Heinrich and the Theory of Accident Causality)
2.2 - Phases of Risk Management
2.2.1 - Risk Analysis
2.2.2 - Risk Assessment and Risk Matrix
2.2.3 - Risk Control

3. Good Laboratory Practices - Basic Standards
3.1 - Clothing and PPE
3.2 - Behavior
3.3 - Organization and Cleanliness
3.4 - Waste Management
3.5 - Work with Flames, Heat, Pressurized and Electrical Equipment
3.6 - Information Management: Safety Data Sheets and Labels
3.7 - Auxiliary Equipment

4. Personal Protective Equipment
4.1 – Hierarchy of risk control measures: Collective protection versus individual protection
4.2 – Characteristics of PPE, legislation, CE marking and PPE categories according to the level of protection required
4.3 – Head protection
4.3.1 – Typical injuries
4.3.2 – Helmets. Structure and functions. Construction materials and instructions for use.
4.4 – Eye protection
4.4.1 – Characteristics and specifications of eye protectors
4.4.2 – Eye protection in the laboratory. Glasses and barriers (Hotes). Contact lenses.

4.4.3 – Work with compressed air
4.5 – Ear protection
4.5.1 – Sound Pressure Level. dB and dB(A) scale. Sound level meters.
4.5.2 – Noise levels and effects.
4.5.3 – Ear protection devices and selection criteria
4.6 – Respiratory protection
4.6.1 – Categories of respiratory protection devices (RPDs) by mode of operation. Filtering RPDs - positive pressure and negative pressure - and self-contained RPDs.
4.6.2 – Self-filtering masks, half-masks and full-face masks
4.6.3 – Particle filters. Protection levels based on legally stipulated emission limit values ??(ELVs)
4.6.3 – Gas and vapor filters. Numerical and color codes based on the substances retained. Classes (retention capacity). Combined filters. Marking
4.6.4 – Calculation of the Nominal Protection Factor (NPF) and the Necessary Protection Level (NPL) based on the ELV. Maximum concentration value
4.6.5 – Self-contained self-contained self-contained breathing apparatus (SCBAs). Criteria for use, advantages and disadvantages.

4.6.6 – Simple or air-pumped non-autonomous insulating respirators. Criteria for use, advantages and disadvantages
4.7 – Hand protection
4.7.1 – Characteristics of gloves according to the type of risk. Classes.
4.7.2 – Glove materials and chemical resistance

5 – Exposure to Chemical Agents
5.1 – REACH and CLP (CRE) Regulations
5.2 – European Chemicals Agency - ECHA
5.3 – Substance (stream), mixture and article, according to REACH
5.4 – REACH regulation processes. SVHC substances
5.5 – Competent national and supervisory authorities within the scope of REACH and CLP
5.6 – CLP and the Globally Harmonised System (GHS)
5.7 – Legal obligations in the supply chain
5.7.1 – Importers and manufacturers. Obligation to register and create Safety Data Sheets (SDS) and Chemical Safety Reports
5.7.2 – Distributors. Information transfer upstream and downstream of the chain
5.7.3 – Downstream Users. Formulators. Industrial users. Professional users. Transfer operators
5.8 – Simple (SDS) and extended (Extended) Safety Data Sheets
5.8.1 – Structure
5.8.2 – Availability
5.8.3 – Chemical Safety Assessment and Chemical Safety Reports. Exposure Scenarios. Operating Conditions and Risk Management Measures
5.8.4 – Internal processing of an SDS or Extended Safety Data Sheet
5.9 – Classification and Labeling – MSDS and labels
5.9.1 – Hazardous material classes according to CLP
5.9.2 – Label elements. H, P and EUH phrases. Signal words. Supplementary information. Pictograms. UFI Codes (Unique Formula Identifier)
5.9.3 – Harmonized toxicological notification
5.9.4 – Methodologies for resolving conflicts in the classification of chemical products
5.10 – Chemical risk assessment
5.10.1 – Assessment of its necessity and aspects to be considered in its execution
5.10.2 – Steps
5.10.3 – Determination of the concentration of chemical agents. Colorimetric tubes. Accredited laboratories
5.10.4 – Chemical agent assessment reports
5.10.5 – Frequency of measurements

6 – Storage of Hazardous Materials – Mobile/Transitable Containers – Relevant Standards
6.1 – Quantities stored
6.2 – Proper use of containers
6.3 – Compatibility between classes of stored materials. Typical errors
6.4 – Separation of chemical products. Retention trays and basins (adequate capacity and construction materials)
6.5 – Construction materials of containers
6.6 – Conservation of containers
6.6.1 – General rules
6.6.2 – Fire-resistant safety cabinets. Characteristics. REI classification (Euroclasses of fire resistance). Rules of use
6.7 – Ventilation and Drainage
6.7.1 – General dilution ventilation. Positive pressure environment and negative pressure environment
6.7.2 – Localized exhaust, laminar flow chambers and hoods
6.8 – Prefabricated containers. Characteristics
6.9 – Work with flammable materials
6.10 – ATEX environments
6.10.1 – Categories of ATEX equipment
6.10.2 – Classification of hazardous areas
6.11 – Signage and rules for occupying storage space
6.12 – Transfer
6.12.1 – Associated risks
6.12.2 – Preventive measures
6.12.3 – Containment devices

Evaluation Methodology
1 - Continuous Assessment:

Written exam with access to printed material. Students who obtain a grade of 10 or higher are exempt from the exam and pass the course.

2 - Assessment by Exam (any time):

Written exam with access to printed material. Students who obtain a grade of 10 or higher pass the course.

Bibliography
- Agência Portuguesa do Ambiente, . (0). Classificação, Embalagem e Rotulagem (CLP). Acedido em 2 de maio de 2022 em https://apambiente.pt/prevencao-e-gestao-de-riscos/classificacao-embalagem-e-rotulagem-clp
- Agência Portuguesa do Ambiente, . (0). REACH. Acedido em 2 de maio de 2022 em https://apambiente.pt/prevencao-e-gestao-de-riscos/reach
- Carvalho, M. e Almeida, T. e Fernandes, A. e Marques, E. e Carneiro, L. (2016). Guia geral para a seleção de equipamentos de proteção individual (EPI). Lisboa: ACT
- Carvalho, M. e Almeida, T. e Fernandes, A. e Marques, E. e Carneiro, L. (2016). Guia geral para o controlo da exposição a agentes químicos. Lisboa: ACT
- Denios, . (0). 12 NORMAS BÁSICAS DE ARMAZENAMENTO DE SUBSTÂNCIAS QUÍMICAS. Acedido em 2 de maio de 2022 em https://www.denios.pt/empresa/revista-tecnica-da-denios/conhecimentos-sobre-armazenamento-de-produtos-quimicos/as-12-normas-de-armazenamento-de-substancias-quimicas

Teaching Method
Expository method, using real-world practical examples to demonstrate the content taught and promote discussion within the class.

Software used in class
Not applicable

 

 

 


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